Abstract: Jiangshui as the experimental material and Lactic acid bacteria( LAB) were isolated and purified on the selective culture medium by spread plate method and streak plate method. The species were identified by combining physiological and biochemical characteristics and molecular biology method. It found that there were various lactic acid bacteria which were classified into 5 genus and 17 species in Jiangshui.17 LAB strains were selected by calcium- dissolving and the strains were used for produced acid,degraded cholesterol and nitrite experiment.The results showed that most of the selected strains had good produced acid,degraded cholesterol and nitrite ability,which Q2,M5,H4,Q7,M2,H1 were better in all aspects,with a potential of development and utilization.