响应面法优化琼脂糖的DEAE-纤维素法提取工艺

Optimization agarose of DEAE-cellulose method extraction technology by response surface method

摘要: 利用DEAE-纤维素法对琼脂糖进行纯化处理,并应用响应面法进行工艺优化。结果表明:DEAE-纤维素的添加量、提取温度、提取时间为影响琼脂糖中凝胶强度及硫酸根含量的主要因素。经响应面法优化得到最优工艺参数:DEAE-纤维素添加量55g,提取温度80℃,提取时间2.5h。在最佳提取工艺的条件下制得的琼脂糖,其凝胶强度达1210g/cm2,硫酸根含量为0.17%;红外光谱分析结果表明自制琼脂糖与对照琼脂糖结构相似。基因组DNA电泳表明其具有良好的电泳性能。

Abstract: Response surface analysis (RSA) was used to optimize process conditions of agarose extraction. The main factors discussed were:DEAE-cellulose content, extraction temperature and extraction time on influencing in gel strength and sulfate radical content. Using the response surface method to optimize the extraction process, the best parameters were the quantity of DEAE-cellulose 55 g, extraction temperature 80℃ and extracting time 2.5h. With the optimized parameters, the sulfate radical content and gel strength of agarose extracted were 0.17% and 1210g/cm2, respectively. IR spectrum analysis showed that the agrose was similar to standard agarose. The genomic DNA electrophoresis proved that it had a good electrophoretic performance.