Abstract: In this paper, the volatile metabolite spectrum ( MVOCs) of Vibrio parahaemolyticus, Escherichia coli and Staphylococcus aureus were detected by the combination of SPME- GC- MS techniques.And the change trends of MVOCs and unique metabolites were also studied. The results showed that, the 65μm PDMS / DVB fiber head had best extraction effectin 30 min and the MVOCs spectrum of 3 kinds of pathogens varied with the incubation time.The peak numbers of V.parahaemolyticus reached the maximum in 30 h, which was 28 and 31, respectively and the characteristic ingredient was 2- chloro- 4- ( 4- methoxyphenyl) - 6- ( 4- nitrophenyl) pyrimidine, tert- butyl hydroperoxide and 3- methyl-1- butyl alcohol.The number of peaks of E.coli and S.aureus reached the maximum in8 h and 12 h, which were 47 and 42, and the the characteristic ingredient were cyclododecane, 2- methyl-1- butanol and indolizine, 3- methyl- butyric acid and 4- methoxy- benzonitrile, respectively.The results provided the basis for volatile markers and rapid detection of three foodborne pathogens.