表面活性剂辅助酶法提取黄芪黄酮的工艺优化

Process optimization of surfactant assisted enzyme extraction of Astragalus flavonoids

摘要: 以乙醇为提取剂,采用表面活性剂辅助纤维素酶解法预处理从黄芪中提取黄酮,在单因素实验结果基础上,利用Box-Behnken的中心组合实验设计,运用响应面分析法对影响黄芪黄酮得率的主要因素（溴化十六烷基三甲胺（CTMAB）添加量、提取温度、乙醇浓度）进行优化,得出黄芪黄酮的最佳提取工艺条件为:CTMAB添加量0.37%,乙醇浓度80%,提取温度79℃,在此条件下黄酮得率实测值为4.192%,理论值为4.287%,实测值与理论值相差较小。

Abstract: The flavonoids was extracted by surfactant- assisted enzymatic pretreatment with alcohol as the solvent from Astragalus.Experiment factors and levels were firstly selected by one- factor tests. According to the Box-Benhnken center- united experimental design principles, the method of response surface analysis was adopted to optimize extraction of flavonoids.The results showed that the optimum conditions were: addition of CTMAB 0.37%, concentration of ethanol 80%, temperature 79℃.Yield of that optimized procedure was 4.192%, which was close to the predicted value of 4.287%.