蓝孔地花菌多糖的体外抗氧化活性

Antioxidant activities in vitro of polysaccharide from Albatrellus caeruleoporus

摘要: 采用水提醇沉法提取蓝孔地花菌子实体多糖,并通过DE-52和Sephadex G-100柱色谱进行纯化,得纯化多糖（ACP1）。以还原能力、清除DPPH自由基、羟自由基、超氧阴离子能力和螯合铁离子能力为指标,评价了ACP1的抗氧化活性。结果表明ACP1具有较强的体外抗氧化活性,其活性大小与浓度呈正效应,ACP1的总还原力、清除DPPH自由基、羟自由基、超氧阴离子能力和螯合铁离子能力的IC50分别为0.09、0.27、0.64、0.42、0.62mg/m L。说明蓝孔地花菌多糖可以作为天然的抗氧化剂应用于食品和药品行业。

Abstract: ACP1 was extracted by water extraction and alcohol precipitation, then purified by DEAE- 52 and Sephdex G-100 column chromatography from fruiting bodies of Albatrellus caeruleoporus.The antioxidant activities of ACP1 were investigated by reducing power, DPPH, hydroxyl, superoxide anion radical scavenging, and chelating ability assays. The results showed that ACP1 had in vitro strong antioxidant activities, whose activities were positively correlated with concentration. The IC50 of reducing power, scavenging DPPH radical, hydroxyl radical, superoxide anion and chelating ability were 0.09, 0.27, 0.64, 0.42 and 0.62 mg / m L.It indicated that the ACP1 may be used as natural antioxidant additive in foods and medicines.