高产洛伐他汀红曲霉复合诱变育种

Screening of high lovstatin producing strains with compound mutagenesis by UV and DES

摘要: 为了提高洛伐他汀的产量,对红曲霉进行了紫外线与硫酸二乙酯复合诱变处理,并利用粗糙脉孢菌对洛伐他汀的敏感性进行初筛,再通过HPLC法测定其发酵液中洛伐他汀的产量进行复筛。最终得到一株稳定高产的目的菌株,命名为Monascus sp.UV-D-9。其洛伐他汀产量达到（0.289±0.02）mg/m L,相对于出发菌株提高了82.9%。Monascus sp.UV-D-9是一株优良的洛伐他汀生产菌株,有一定的应用价值。

Abstract: In order to improve lovstatin yield of Monascus sp.3.00567, the starting strains were mutagenized by UV and DES, which were primaryly srecreened based on the sensitivity to lovastatin of Neurospora crassa. The secondary screening was made through determinated lovstatin yield of fermentation liquid by HPLC. A high and stable yield mutant was obtained and named Monascus sp.UV-D-9. The lovstatin yield of Monascus sp.UV-D-9 was (0.289±0.02) mg/m L, which increased 82.9% compared with the original strain. Monascus sp.UV-D-9 had certain value as an excellent lovastatin producing strain.