HPLC-ELSD法测定冰酒中的糖类含量
Determination of sugars in five types of ice wine by high performance liquid chromatography ( HPLC) with evaporative light- scattering detector ( ELSD)
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摘要: 建立HPLC-ELSD法测定冰酒中糖类含量的方法,应用高效液相色谱仪,NH2柱（5μm,4.6mm×250mm）,柱温40℃,流动相:乙腈∶水=80∶20,流速:0.8m L/min,蒸发光散射检测器检测,检测器温度为40℃,20μL进样。测得六种冰酒中主要糖类为果糖和葡萄糖,果糖含量大于葡萄糖含量,且各品种中的果糖和葡萄糖含量差别相对较大,6次平行进样,相对标准偏差为0.7%2.1%,葡萄糖和果糖的加标回收率94.7%103.4%,该方法具有准确,快速,操作简单等特点,可用于冰酒中糖类含量的检测。Abstract: A method was established to determine sugars in wine by using HPLC ( High Performance Liquid Chromatography) with ELSD ( evaporative light- scattering detector) .The samples were separated on a NH2 ( 5μm4.6mm × 25cm) chromatographic co Lumn.The flowing phase was C2H3 N ∶ H2 O = 80 ∶ 20 and the velocity was0.8m L / min. The temperature in column and detector was 40℃ and the input volume was 20μL. Experimental result showed that there were almost two types of sugar in six types of ice wine: fructose and glucose.Fructose content was more than glucose levels, and fructose and glucose content in each type of ice wine were difference.The relative standard deviation of this method was 0.7% 2.1% ( n = 6) and the recovery was 94.7% 103.6%.The method was accurate and simple, and was suitable for rapid detection glucose and fructose in ice wine.
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