响应面法优化超声辅助提取野木瓜多酚工艺
Optimization of ultrasonic- assisted extraction of polyphenols from Stauntonia chinensis using response surface methodology
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摘要: 以野木瓜为原料,采用响应面法优化超声波辅助提取野木瓜多酚的工艺条件。在单因素实验的基础上,采用Box-Behnken设计,对超声时间、超声温度、丙酮体积分数、料液比等工艺参数进行优化。结果表明,野木瓜多酚提取的最佳工艺条件为超声时间20min,超声温度63℃,丙酮体积分数58%,料液比1∶20（g/m L）。经验证,该条件下野木瓜多酚的得率为8.402mg/g,与预测值8.426mg/g的相对误差为0.3%。该法所得的优化提取条件工艺参数可靠,可行性强,可为野木瓜中多酚产品的开发利用提供科学依据。Abstract: The ultrasonic- assisted extraction of polyphenols from Stauntonia chinensis was optimized using response surface methodology. Based on the single factor experimental analysis, Box- Behnken experimental design was used to explore the optimal processing conditions to extract polyphenols from Stauntonia chinensis including ultrasonic treatment time, ultrasonic temperature, acetone concentration and solid- to- solvent ratio. The results showed that the optimal extraction conditions were ultrasonic treatment time of 20 min, ultrasonic temperature of 63℃, acetone concentration of 58% and solid- to- solvent ratio of 1∶20 ( g / m L) .Through certification, the extraction yield of polyphenols from Stauntonia chinensis was 8.402 mg / g under the optimal conditions. In conclusion, the optimized extraction process is reliable and can be utilized to develop products based on Stauntonia chinensis polyphenols.
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