滩涂植物碱蓬籽中总酚的提取工艺优化
Extraction of total phenols from Suaeda glauca Bge seed and optimization of extraction process by RSM
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摘要: 以盐城滩涂湿地物种碱蓬为原料,乙醇为提取溶剂,选用超声辅助的提取方法研究碱蓬籽中的总酚提取工艺并对工艺进行优化。在单因素实验的基础上,确定提取温度、提取时间、料液比及乙醇的体积分数四个主要因素,研究对响应值总酚的提取量。采用响应面分析法中的Box-Behnken Design中心组合法设计实验工艺,通过方差分析确定回归模型及分析各因素的交互作用。优化的最佳工艺条件为:以80%乙醇溶液为提取剂,提取时间为80min,提取温度为80℃,料液比为23g/m L,此时理论预测提取量为996.94μg/g。在此条件下进行三次平行验证实验测得碱蓬籽中的总酚提取率为986.03μg/g,RSD%为1.41,说明实验得到的回归模型用来研究碱蓬中的总酚含量是准确的和科学的,具有一定的参考价值。Abstract: Total phenols from Suaeda glauca Bge Seed growing on beach was extracted using ethanol as solvent by ultrasonic- assisted extraction ( UAE) method, and extraction process was optimized by Box- Behnken Design in response surface methodology ( RSM) based on single factor experiment, which investigated the influence of temperature, ultrasonic time, solid- liquid ratio and volume fraction of ethanol on total phenolic content ( TPC) . The regression model was built up and interactions between factors were analyzed. The results showed that optimal extraction conditions were 80℃, 80 min, 23g / m L of solid- liquid ratio and 80% of ethanol solvent, and regression model was obtained with 996.94μg / g of theory content of total phenols.Under the optimum conditions, the average value of TPC was 986.03μg / g, RSD% = 1.41.This indicated that the regression model was accurate and reliable for investigation on TPC.
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