副溶血性弧菌噬菌体的分离及其在即食虾中的应用

袁琳; 张昭寰; 崔泽林; 郭晓奎; 赵勇

doi:10.13386/j.issn1002-0306.2014.21.030

副溶血性弧菌噬菌体的分离及其在即食虾中的应用

Isolation of Vibrio parahaemolyticus phage and its application in ready-to-eat shrimp

摘要

摘要: 为了研究噬菌体作为天然生物防控剂应用于食品中的前景,本实验从水产品市场的污水中采用双层平板法分离纯化出一株烈性副溶血性弧菌噬菌体SHOU24,测定其生理特性,并探讨其在即食虾中的抑菌效果。结果表明:该噬菌体只对含有毒力基因tdh的副溶血性弧菌有裂解能力。电镜观察其形态表明该噬菌体属于长尾噬菌体科。SHOU24的最适p H为7~8。在50℃温度以下,存活率很高。抑菌实验结果表明该噬菌体在常温条件下对即食虾中副溶血性弧菌的生长具有良好的抑制作用,能使副溶血性弧菌的菌浓度下降1log CFU/g。本研究为副溶血性弧菌噬菌体SHOU24作为抑菌剂应用于食品中提供了一定的理论基础。

Abstract: Aimed at using bacteriophage as natural anti- bacteria, this study isolated a lytic Vibrio parahaemolyticus phage through sewage from aquatic market by double- layer method and it was designated as SHOU24, and p H and thermal stability were tested. Phage SHOU24 was specific lytic to Vibrio parahaemolyticus with tdh gene. The phage belonged to Siphoviridae by transmission electron microscope ( TEM) . The most suitable p H for phage SHOU24 was 7⁸ while it could live at p H between 3¹1.The survival rate was high under 50℃ condition.In antibacteria on ready- to- eat shrimp experiment, the bacteria amount dropped by 1log CFU / g at 25℃. This study provided theory foundation for using phage SHOU24 as bacterial biocontrol agent.