Abstract: Fructooligosaccharides are produced by the inulin cut with endoinulinase and microorganism is the main sources for endoinulinase.In present work, 14 strains microorganism producing inulinase were acquired from Yalu River seacoast wetland samples with selective medium containing inulin as a sole carbon source.Meanwhile, three strains with high endoinulinase activity were obtained, and their enzyme activities were 14.13, 18.24 and 28.52 U / m L, respectively.Through morphological and analysis of the 16 S rRNA revealed that the strain S502, S503 and S505 identified as Streptomyces malachitofuscus, Micromonospora echinospora and Streptomyces carpaticus, respectively.The endoinulinase activity by actinomycete is higher than that reported in the literatures.Furthermore, as a new potential candidate for industrial endoinulinase production.