Abstract: In the present study, taking two batches with low ethanol accumulation under 2g / L as control group, effect of long- term ( > 4h) and instantaneous high ethanol concentration ( > 10 g / L) on porcine interferon αproduction and activities of key enzymes in carbon metabolism was discussed. As a result, long- term high ethanol concentration severely deteriorated specific activities of the enzymes, and p IFN- α expression level was decreased about 4 ~ 12 folds. Similarly, instantaneous high ethanol concentration also deteriorated specific activities of the enzymes, and p IFN- α expression level was decreased about 8 folds.