Abstract: Poly (lactic acid) (PLA) nanofiber films and TiO2/PLA composite nanofiber films were prepared by electrospinning technology, and were used as new fresh-keeping materials. The strawberries were packaged with PE cling films, pure PLA nanofiber films and TiO2/PLA composite nanofiber films respectively. The change appearance, weight loss rate, decay index, titratable acidity content, ascorbic acid content (VC) of strawberries were observed and determined during storage in order to study the fresh-keeping effect of packaging materials.The results indicated that the effect of TiO2/PLA composite nanofiber films on the preservation of strawberries was optimal. TiO2/PLA composite nanofiber films could decrease the weight loss and decay index, and delay the decline of titratable acidity content and ascorbic acid content (VC) of strawberries to some extent, and contributed to prolong the storage period of strawberries.