Abstract: In this study, the protein was extracted from corn bran and alkali extraction was selected to optimize by response surface method. The effects of extraction pH, extraction time, extraction temperature, solvent/material ratio and on extraction efficiency were explored. Based on the single-factor experiments, the three key factors, including pH, temperature, solvent/material ratio were selected to design the three-factors-three-levels Box-Behnken experiments design. As a result, a mathematical model was established and the optimum protein extraction condition of corn bran were:extraction time 1.5h, extraction temperature 46℃, pH10, material/solvent ratio 1∶11, resulting in an extraction yield of 79.15%.