Abstract: The cell growth and nutritional components in Chlorella sorokiniana under different culture medium, different nitrogen sources, concentration of nitrogen and concentration of phosphorus was investigated. Results showed that the most adequate culture medium was BBM. There was no significant difference to the content of lipid, protein and pigment under different culture medium, the highest content of polysaccharose was 44.46% in the BG-11 medium. The best nitrogen source was glycine, with which the biomass, content of lipid, protein and polysaccharose were higher than other nitrogen sources. The optimum concentration of nitrogen source was13.33mmol/L. Without any nitrogen sources, the biomass, content of lipid, protein, and pigment were least, but the polysaccharose content was highest, which was 2 folds of other groups. The optimum concentration of phosphorus was 1.72mmol/L. With increasing concentration of phosphorus, the biomass, lipid and protein content of Chlorella sorokiniana were increasing at first and then decreasing, and polysaccharides and pigment content were decreasing slightly.