Abstract: A high-performance liquid (HPLC) chromatography method was established to analyze the oil load efficiency of VEemulsion, and the obtained data could be used to evaluate the stability of emulsion. The effect of pH, temperature, ion concentration and free-OS concentration on oil load efficiency of VEemulsion were examined. The results showed that the optimal pH was 7, and the stability of emulsions decreased along with the increasing temperature, ion concentration and free-OS concentration. Response surface methodology was further applied to study the formation of VEemulsion. And the relative degree of influence of all environmental factors on its oil load efficiency was that temperature>NaCl concentration>free-OS concentration>pH.