Abstract: The diabetic mouse models were established by intravenous injection of alloxan, the levels of blood sugar, hepatic glycogen and TG, TCH, MDA and SOD in serum were detected to evaluate hypoglycemic activity, lipid metabolism and oxidative stress of blackberry seed. The results showed that low dose group of petroleum ether extract could significantly decrease the content of liver glycogen ( p < 0.05) , and increase the contents of TG ( p <0.01) , TCH ( p < 0.05) and MDA ( p < 0.05) .High dose group of ethyl acetate extract could significantly decrease the content of liver glycogen ( p < 0.05) , and decrease the contents of TG ( p < 0.05) and TCH ( p < 0.05) .Medium dose group of ethyl acetate extract could significantly increase the content of liver glycogen ( p < 0.01) , and decrease the contents of TG ( p < 0.05) and TCH ( p < 0.05) , and increase the level of SOD ( p < 0.05) .High dose group of blackberry seed oil could significantly decrease the contents of TG ( p < 0.05) and TCH ( p < 0.05) . Low dose group of blackberry seed oil could significantly increase the content of liver glycogen ( p < 0.05) , and decrease the content of TCH ( p < 0.05) .It provided evidence that the petroleum ether extract, ethyl acetate extract and oil of blackberry seed had no significant hypoglycemic effect, and could promoted hepatic glycogen synthesis and inhibited excessive degradation, rectified the derangement in lipide metabolism and strengthened antioxidant defense system.