蜂王浆主蛋白提取工艺优化及质量分析

于张颖; 肖发; 柳丹丹; 翟量; 沈立荣

doi:10.13386/j.issn1002-0306.2014.19.031

蜂王浆主蛋白提取工艺优化及质量分析

The optimization and quality analysis on extraction of the major royal jelly proteins ( MRJPs) from royal jelly

摘要

摘要: 用离心法从鲜王浆中提取蜂王浆主蛋白,进行液料比、pH、抽提时间和离子强度对MRJPs提取率影响的正交实验,并作MRJPs蛋白质量、副产物王浆酸和总糖回收率分析。实验结果表明:影响MRJPs提取率的因素从大到小依次为离子强度>pH>抽提时间>液料比,在最佳提取条件（液料比8mL/g、pH8、抽提8h,离子强度0.5mol/L）下,MRJPs提取率为81.14%。提取的MRJPs蛋白SDS-PAGE电泳和ELISA分析显示,其分子量在42.1⁹7.4ku,纯度71.7%。副产物分析显示,王浆酸和总糖回收率分别为2.91%和1.26%。该结果为王浆活性蛋白开发和副产物综合利用提供了科学依据。

Abstract: Major Royal Jelly Proteins ( MRJPs) was extracted by centrifugation from royal jelly ( RJ) .The orthogonal experiment optimized with four parameters, ratio of solution to royal jelly, pH, extracting time and ionic strength was implemented.The quality of MRJPs, the trans-10-hydroxy-decenoic and total sugar in by-product were analyzed.Orthogonal experiment showed the factors affecting MRJPs extraction rate in descending order of size were: ionic strength > pH > extracting time > ratio of solution to RJ.81.14% of MRJPs was obtained from RJ under the optimum extraction conditions ( the ratio of solution to RJ 8mL /g, pH8, extracting time 8h, ionic strength 0.5mol /L) .Analysis of SDS-PAGE and ELISA of extracted MRJPs showed that it possessed molecular weight ranged from 42.1 to 97.4ku, and that its purity reached 71.7%.The by- product contained 2.91% of trans-10- hydroxy-2- decenoic acid and1.26% of total sugar, respectively. The results provided essential scientific knowledge for development of MRJPs and utilization of its by-products.