Abstract: The traditional surface aerobic fermentation was replaced of a packing- tower fermentation system by design.The device could keep the cellulose membrane integrity and stable and feed batch operation easily during fermentation.Several kinds of feeding operations and comparisons between single- batch fermentation and fed-batch fermentation were made in the experiment. The results showed the fermentation system could replace the traditional surface fermentation system.Compared with single batch fermentation, ethanol feeding batch operation in the device and static fermentation system could improve total acidity by 71.1% and 87.5% which proved to be an effective way to improve the fermentation total acidity.The initial ethanol concentration was negative correlative with cellulose production while positive correlative with acid production, and ethanol could transfer to acetic acid as much as possible on the concentration of 5.0% ( V/V) .Raising the feeding frequencies on the 5.0% ( V/V) ethanol concentration could keep the ADH activity in higher degree to improve the velocity of acid production, and feeding frequency at every 12 h obtained maximum average ADH activity of 0.051 U /g, but invalid in the maximum total acidity.