食源性蛋白酶抑制物的筛选及对白鲢鱼糜凝胶特性的影响
Screening of foodborne protease inhibitor and effect on gel properties of surimi from sliver carp
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摘要: 为筛选食用安全并具有蛋白酶抑制作用的食源性物料,本文以白鲢鱼糜为研究对象,在明确了温度、水分对凝胶劣化过程影响的基础上,采用质构分析、持水率测定等评价方式对多种食源性物料的添加效果进行对比,并对有效物料进行了添加量和添加形式的研究。结果显示:60℃为白鲢鱼糜凝胶劣化的最适温度,凝胶强度随水分含量的增加而降低,当水分含量大于81.0%(w/w)时下降趋势尤为明显;鱼籽对蛋白酶的水解过程有浓缩依赖性抑制作用。添加0.5%(w/w)新鲜鱼籽的鱼糜制品凝胶强度达到峰值,较空白样品提高了27.97%;冷冻干燥对鱼籽的抑制效果影响较小。Abstract: In order to screen out the safety and low- priced foodborne materials, which had a function of protease inhibition.Through adding different foodborne material into silver carp surimi, which had a same total moisture content.The add effect copared by the use of texture analysis, determination and water-holding evaluation based on a clear modori temperature and the effect of moisture content on gelling properties were investigated.We also had a futher study on the optimal addition amount and add form in low concentration.The results showed that 60℃was the optimum gel deterioration temperature of silver carp surimi, the gel strength decreased with the increase of water content and particularly when the added water was greater than 81.0% ( w /w) . Fish roe showed inhibitory activity against proteolysis in a concen- tration- depen dent manner.When added 0.5% ( w /w) of fresh, the gel properties of surimi products were improved with a increasing gel strength by 27.97%.The activity of protease inhibitors was slightly affected in Freeze-drying process.