响应面法优化鲁氏接合酵母产MAP酶条件

Optimization of MAPase producing from Zygosaccharomyces rouxii by response surface method

摘要: 为研究产MAP酶的最佳培养条件,以鲁氏接合酵母为原料,MAP酶为响应值,在单因素实验的基础上根据Box-behnken实验设计原理,采用三因素三水平的响应面分析法对MAP酶培养条件进行优化,通过响应面实验,建立了MAP酶含量与三个因素变化的二次回归方程。同时根据回归模型进行了计算机模拟实验并绘制了曲面图,探索MAP酶随主要因素水平的变化规律与优化点。结果表明,鲁氏接合酵母产MAP酶最佳培养条件为:温度27℃、盐浓度9.28%、培养时间80.4h,此培养条件下MAP酶浓度3.189ng/mL。

Abstract: Using MAPase concentration as the response value, Zygosaccharomyces rouxii as the raw material to study the optimal MAPK conditions.Based on the result of single-factor test, response surface methodology with 3factors and 3 levels was adopted according to box- behnken experiment design principle. Two regression equations for the influence relationship of MAPase concentration and three varietals factors were established.On the basis of regression models, the results with surface were simulated to give optimized result and trends of the main factors on the educing sugar concentration.The optimal MAPK conditions were as follows: the temperature was 27℃, salt Concentration 9.28%, time 80.4 hours. Under this condition the MAPase concentration was3.189 ng /mL.