Abstract: Duck liver was selected as raw material, and lecithin was extracted with ultrasound-assisted method.The ultrasonic temperature, ultrasonic time, ethanol concentration, ethanol level and ultrasonic power were controlled by single factor experiment, and subsequently response surface methodology was conducted to optimize ultrasonic assisted extraction based on lecithin extraction ratio.The experiment results showed that the optimum condition of ultrasonic- assisted extraction of lecithin from duck liver was achieved at the ultrasonic temperature of 35℃, ultrasonic time of 43 min, ethanol concentration of 95% and ethanol volume of 17 mL. The validation experiment showed that the duck lecithin extraction ratio was 76.34%, which indicated a high extraction ratio of lecithin could be obtained, and these extraction conditions could be used for industrial application.