Abstract: Protein phosphorylation is the most common and extensive post-translational modification of proteins.The latest research methods including the enrichment, site analytical and quantitative analytical methods of phosphorylated proteins were summarized in this paper. The effects of protein phosphorylation on meat quality were investigated based on the relationship between protein phosphorylation and glycolysis, muscle contraction and protein degradation. The direction of phosphoproteomics development and research of protein phosphorylation in meat science field were discussed.