Abstract: Ultrasonic treatment was used to extract β-glucan from oat. Congo red method was used to calculate the yield of β-glucan. The optimal extraction conditions by single factor test and orthogonal test were obtained.The comparison of the properties of β-glucan between by ultrasonic treatment extraction and by water method extraction was carried out. The results showed that the optimal extraction conditions were as following:the ratio of water to material 20 ∶1, the ultrasonic power 720 W, the ultrasonic time 35 min, the temperature 50℃ and the pH10, under this condition, oat β-glucan yield could reach up to 4.09%, however, the yield of β-glucan by water method only could reach up to 3.05%. Compared to β-glucan by water method, the properties of β-glucan by ultrasonic treatment such as water-binding and oil-binding capacity were improved.