灵芝菌丝体中灵芝酸T、R和S的HPLC测定方法的建立和应用

Quantitative analysis of ganoderic acid T, S and R in Ganoderma mycelium by HPLC

摘要: 采用YMC C18柱（250mm×4.6mm,5μm）,以0.5%冰醋酸溶液（A）-甲醇（B）为流动相梯度洗脱（0²0min,85%B→100%B;20³0min,100%B）,检测波长在245nm,流速1mL/min,柱温为30℃的条件下,用HPLC考察灵芝酸T、灵芝酸S、灵芝酸R在不同灵芝菌丝体中的含量差异。结果表明,不同种类的灵芝菌丝体中三种灵芝酸成分的含量存在显著的差异,p=0.0003,而且此方法操作简便,稳定性和重复性良好,适用于同时快速检测灵芝菌丝体中三种灵芝酸含量。

Abstract: An HPLC-based method for the accurate quantitative analysis of GA-T, GA-S and GA-R in different Ganoderma mycelium was described. The HPLC method was performed on YMC C18column (250mm×4.6mm, 5μm) , with 0.5% acetic acid (A) -methanol (B) as mobile phase (0~20min, 85%B→100% B, 20~30min, 100% B) .UV detection wavelength was 245 nm. The flow rate was 1mL/min. The column temperature was 30℃. The result suggested significant differences in analyte ganoderic acid content among the different G. lucidium strains, and the significance level was 0.0003. The method was proved to be convenient, stabilized and repeatable, so it could be used for the quantitative analysis of three ganoderic acids in Ganoderma mycelium.