草鱼鱼鳞酶溶性胶原蛋白肽脱腥脱苦工艺研究

鉏晓艳; 熊光权; 李新; 耿胜荣; 乔宇; 江洪有; 廖涛

doi:10.13386/j.issn1002-0306.2014.15.042

草鱼鱼鳞酶溶性胶原蛋白肽脱腥脱苦工艺研究

Study on debittering of papain soluble collagen peptide from Grass carp scale

摘要

摘要: 以草鱼鱼鳞提取的酶溶性胶原蛋白肽（papain soluble collagen peptide,PSCP）为研究对象,研究不同截留分子量纳滤膜对PSCP回收率的影响;并以正交实验L9（34）研究了不同β-环糊精添加量、水浴时间、水浴温度对PSCP腥苦味及回收率的影响;最后分析了PSCP中氨基酸的含量和比例。结果显示,纳滤膜截留分子量为400⁶00u时,PSCP回收率最小（16.71%）,推测PSCP分子量介于300⁶00u之间。β-环糊精最佳包埋条件为:添加量4%、水浴温度70℃、水浴时间40min,在此条件下鱼鳞胶原蛋白肽的回收率为92.21%,感官评分为9.30,脱腥脱苦效果最好。PSCP氨基酸分析结果显示,有16种氨基酸含量大于1%,共占总氨基酸含量的99.07%,其中羟脯氨酸含量大于8%。该方法及优化后的工艺参数为工业化生产提供了依据。

Abstract: In this paper, papain soluble collagen peptide ( PSCP) was used as research object, which extracted from grass carp scale, to study PSCP recovery rate under nanofiltration membranes with different molecular weight cut off ( MWCO) .Orthogonal experiment ( L9 ( 34) ) was used to research the influence of β-cyclodextrin dosage, bath time and bath temperature on fishy bitterness and PSCP recovery rate. Finally, content and proportion of PSCP amino acid were analyzed. The results showed that, recovery rate ( 16.71%) was lowest when the nanofiltration membrane MWCO was about 400~600u, suggesting that the molecular weight of PSCP might between 300~600u.The optimal embedding conditions were cyclodextrin amount of 4%, bath temperature of 70℃, bath time of 40 min.Under this condition, the best deodorization effects were achieved with PSCP recovery rate was 92.21% and sensory score was 9.30.The analysis results of PSCP amino acid showed that there were 16 kinds of amino acid content of more than 1% in PSCP, total content of 99.07% in total amino acid, in which the hydroxyproline ( Hyp) content was higher than 8%.The method and process parameters optimized could provide a basis for industrial production.