聚乙二醇-硫酸铵双水相体系分离纯化黄花蒿黄酮的研究

Separation and purification of flavonoids from Artemisia annua L.by polyethylene glycol-ammonium sulfate aqueous two-phase system

摘要: 采用聚乙二醇-硫酸铵双水相体系分离纯化黄花蒿黄酮。通过单因素实验考察聚乙二醇平均分子量、（NH4）2SO4质量分数、聚乙二醇质量分数、pH和体系温度等对分配系数、萃取率的影响,并通过正交实验对萃取工艺条件进行进一步的优化,获得最佳工艺条为:双水相体系组成30%PEG600-25%（NH4）2SO4,体系温度45℃,体系pH为3。在此条件下,黄花蒿黄酮主要分布在上相,分配系数为21.86,萃取率达到96.33%。

Abstract: An aqueous two- phase system of PEG- ( NH4) 2SO4was used to extract flavonoids from the crude ethanol extract of Artemisia annua L..The effects of average molecular weight of PEG, mass percent of ( NH4) 2SO4, mass percent of PEG, pH and temperature on partition coefficient and extraction rate of flavonoids were explored by single factor experiments, then the extraction condition of flavonoids was further optimized by orthogonal method.Then the optimum conditions were obtained as follows: An aqueous two- phase system of 30% PEG600 and 25% ( NH4) 2SO4, pH of 3 and temperature of 45℃.Under the optimal extraction conditions, flavonoids from Artemisia annua L.was mainly distributed in the upper phase with a partition coefficient of 21.86 and the extraction rate of 96.33%.