Abstract: The examination effect of Salmonella in food with GB 4789.4-2010, API 20 E, VITEK and mini VIDAS was compared with the following aspects: the properties of Salmonella chromogenic medium, sensibility, specificity, anti-interference and time consuming.The separation effect of chromogenic medium impacted the examination effect directly.The results indicated BS and RS had better selectivity, while Salmonella grew better on XLD and BS.The detection limits of mini VIDAS was 105 CFU /mL. SX2 was better than TTB and SC as enrichment broth of mini VIDAS.Four methods all could exclude the interference of six negative control strains.When Salmonella mixed with Shigella soonei, XLD and HE could separate suspicious bacteria, other methods reported false negative.