滇重楼多糖不同提取方法的比较研究
Comparative studies on different extraction process of polysaccharide from Paris polyphylla var. yunnanensis
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摘要: 对滇重楼多糖的不同提取工艺进行研究。探讨料液比、提取时间、提取温度和提取次数对多糖得率的影响,在单因素实验的基础上,通过正交实验确定最佳提取工艺条件。结果表明:回流提取的最佳工艺条件为在95℃水浴条件下,以1∶60(g∶mL)的料液比,提取时间1h,提取3次,最优工艺条件下多糖提取率可达18.72mg/g;热水浸提的最佳工艺条件为在85℃水浴条件下,以1∶80(g∶mL)的料液比,提取1.5h,提取2次,最优工艺条件下多糖提取率可达23.84mg/g;超声波辅助提取的最佳工艺条件为在50℃水温条件下,以1∶50(g∶mL)的料液比,提取1.5h,提取2次,最优工艺条件下多糖提取率可达24.32mg/g。3种提取方法对滇重楼多糖提取率影响差异不显著,但超声波辅助提取较热水浸提、回流提取具有能耗低的优点。Abstract: Various extraction process of polysaccharide from Paris polyphylla var. yunnanensis was studied.Effect of the ratio of material to liquid, extraction time, extraction temperature and extraction times on extract rate of polysaccharide was studied. The best extraction process was determined by orthogonal experiment in case of the single factor condition. As a result, the best extraction process for reflux extraction method was to extract 3 times (1h/times) with the ratio of material to liquid for 1∶60 (g∶mL) in 95℃ water bath, and extraction rate of polysaccharide reached 18.72mg/g under the best condition. The best process for the hot water extraction method was to extract 2 times (1.5h/times) with the ratio of material to liquid for 1∶80 (g∶mL) in 85℃water bath, and extraction rate of polysaccharide reached 23.84mg/g under the best condition. The best process for the ultrasonic assisted extraction method was to extract 2 times (1.5h/times) with the ratio of material to liquid for 1∶50 (g∶mL) in 50℃ water bath, and extraction rate of polysaccharide reached 24.32mg/g under the best condition. There was no significant difference among the three extraction methods to extraction efficiency of polysaccharide in P. polyphylla var. yunnanensis.
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