凹土负载壳聚糖对马氏珠母贝糖胺聚糖提取物中镉的吸附研究

Study on adsorption of cadmium in extract of Pinctada martensii glycosaminoglycan by attapulgite-chitosan

摘要: 采用凹土负载壳聚糖对马氏珠母贝全脏器的酶解液进行吸附脱镉研究,通过单因素实验,考察了振荡时间、pH、振荡速度和复合物（凹土-壳聚糖）添加量对糖胺聚糖（GAG）粗品中镉脱除和GAG含量的影响,并利用响应面法优化出镉脱除条件。结果表明:糖胺聚糖中镉脱除的最优条件是振荡时间31.0min,pH7.0,振荡速度143.0r/min,复合物添加量13.0mg/mL,在此条件下脱镉率达到76.9%,GAG含量为47.1%。

Abstract: Absorption of the cadmium of hydrolyzate from Pinctada martensii whole organ by attapulgite-chitosan complex was studied. The effects of vibration time, pH, vibration speed and compound dosage (attapulgitechitosan) of attapulgite-chitosan on cadmium removal rate and GAG yield were investigated through single factor assay, and the conditions of removing cadmium were optimized by response surface method. The optimum conditions of removing cadmium in GAG were 143.0r/min of speed of vibration and pH7.0, processing for 31.0min with 13.0mg/mL dosage of attapulgite-chitosan. Under this condition, cadmium removal rate reached76.9%, GAG yield reached 47.1%.