杏鲍菇产漆酶培养条件优化及其对菲的降解特性研究

Optimization of culture conditions of Pleurotus eryngii for laccase production and its degradation characteristics of phenanthrene

摘要: 在单因素实验的基础上,采用Box-Behnken实验设计方法,以漆酶酶活为响应值,对杏鲍菇发酵产漆酶培养条件进行优化,并经实验验证模型的可行性。最终得到优化后的产酶条件为:转速131r/min,装液量32mL,温度27.6℃;在此条件下杏鲍菇对菲具有较高的降解率,培养12d后,菲降解率达82.86%。

Abstract: Based on the single factor experiment, using Box-Behnken experimental design method, the enzyme activity of laccase as response value, the fermentation conditions were optimized and the feasibility of experiment model was validated. The results indicated optimal ingredients including:the speed at 131r/min, temperature at27.6℃ and shake loading at 32mL/100mL. Under the optimal conditions, the strain of Pleurotus eryngii had higher degradation rate of phenanthrene. The degradation rate of phenanthrene reached 82.86% at the 12th day.