王不留行多糖提取工艺研究及其含量测定

Study on extraction process and content determination of polysaccharides from Semen vaccaria

摘要: 采用水提醇沉提取工艺,运用单因素实验和正交实验,以多糖提取率为评价指标,确定王不留行多糖的最佳提取工艺条件。多糖含量测定方法采用酶解-苯酚-硫酸法。结果表明,王不留行多糖最佳工艺条件为提取温度100℃,提取时间2h,水料比10∶1（mL/g）,提取次数为3次。在此优化条件下的多糖提取率为29.32%。王不留行经清炒炮制后,多糖提取率降为22.15%。

Abstract: The extraction of bioactive polysaccharide in Semen vaccaria was studied in this paper. The water extract and alcohol precipitate method was used for the extraction of polysaccharides and was optimized by single factor and orthogonal experiments. The polysaccharide content was determined by phenol-sulfuric acid method after enzymatic degradation. The optimum extraction condition was obtained as follows:100℃, extract for three times with 2 hours and water-material ratio of 10∶1 (mL/g) for each time. Under the optimal conditions, the polysaccharide yield was 29.32%. The polysaccharide yield for the fried Semen vaccaria was 22.15%.