• EI
  • Scopus
  • 中国科技期刊卓越行动计划项目资助期刊
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国精品科技期刊
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国科技核心期刊CSTPCD
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020
李雪萍, 李建宏, 孟宪刚, 谢佳佳. 西北浆水中兼性厌氧菌的分离与鉴定[J]. 华体会体育, 2014, (12): 213-217. DOI: 10.13386/j.issn1002-0306.2014.12.038
引用本文: 李雪萍, 李建宏, 孟宪刚, 谢佳佳. 西北浆水中兼性厌氧菌的分离与鉴定[J]. 华体会体育, 2014, (12): 213-217. DOI: 10.13386/j.issn1002-0306.2014.12.038
LI Xue-ping, LI Jian-hong, MENG Xian-gang, XIE Jia-jia. Isolation and identification of facultative anaerobes from northwestern jiangshui[J]. Science and Technology of Food Industry, 2014, (12): 213-217. DOI: 10.13386/j.issn1002-0306.2014.12.038
Citation: LI Xue-ping, LI Jian-hong, MENG Xian-gang, XIE Jia-jia. Isolation and identification of facultative anaerobes from northwestern jiangshui[J]. Science and Technology of Food Industry, 2014, (12): 213-217. DOI: 10.13386/j.issn1002-0306.2014.12.038

西北浆水中兼性厌氧菌的分离与鉴定

Isolation and identification of facultative anaerobes from northwestern jiangshui

  • 摘要: 采用多点采样法获取西北不同地区的浆水为实验材料,采用改良的焦性没食子酸法和平板涂布法分离并纯化其中的兼性厌氧微生物,共得到17株菌株,并对纯化后的各菌株进行菌落形态观察、革兰氏染色、生化特性研究以及16S rDNA鉴定。结果发现,浆水中存在多种兼性厌氧菌株,其大多为乳杆菌属(Lactobacillus)或双歧杆菌属(Bifidobacterium)等益生菌,但其中还存在部分杂菌和有害菌;另外还发现不同地区的浆水中兼性厌氧菌种类差别较大。 

     

    Abstract: Jiangshui from different regions of northwestern China was obtained with multiple points sampling method and was used as the experimental material. The improved pyrogallic acid and spread-plate method were applied for separating and purifying of facultative anaerobic microorganism with getting 17 strains. The morphologic observation and the research of gram staining and biochemical characteristic, together with identification of 16 S rDNA were used to analyze the purified strains. The major finding was that there were different sorts of facultative anaerobesin the Jiangshui, and most of them belong to Lactobacillus, Bifidobacterium or the other probiotics. And there also existed part of sundry bacteria and harmful bacteria. In addition, it was found that there was a significant difference of the kinds of the facultative anaerobic microorganism in Jiangshui produced in different places.

     

/

返回文章
返回