西北浆水中兼性厌氧菌的分离与鉴定
Isolation and identification of facultative anaerobes from northwestern jiangshui
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摘要: 采用多点采样法获取西北不同地区的浆水为实验材料,采用改良的焦性没食子酸法和平板涂布法分离并纯化其中的兼性厌氧微生物,共得到17株菌株,并对纯化后的各菌株进行菌落形态观察、革兰氏染色、生化特性研究以及16S rDNA鉴定。结果发现,浆水中存在多种兼性厌氧菌株,其大多为乳杆菌属(Lactobacillus)或双歧杆菌属(Bifidobacterium)等益生菌,但其中还存在部分杂菌和有害菌;另外还发现不同地区的浆水中兼性厌氧菌种类差别较大。Abstract: Jiangshui from different regions of northwestern China was obtained with multiple points sampling method and was used as the experimental material. The improved pyrogallic acid and spread-plate method were applied for separating and purifying of facultative anaerobic microorganism with getting 17 strains. The morphologic observation and the research of gram staining and biochemical characteristic, together with identification of 16 S rDNA were used to analyze the purified strains. The major finding was that there were different sorts of facultative anaerobesin the Jiangshui, and most of them belong to Lactobacillus, Bifidobacterium or the other probiotics. And there also existed part of sundry bacteria and harmful bacteria. In addition, it was found that there was a significant difference of the kinds of the facultative anaerobic microorganism in Jiangshui produced in different places.