Abstract: A new pretreatment method, microbial fermentation of vegetable oil deodorizer distillate (VODD) , was tried in this work. A yeast strain Candida tropicalis1253 was screened, which could utilize fatty acids as carbon source for cell growth, and was fermented soybean oil deodorizer distillate (SODD) . During the fermentation, fatty acids in SODD were utilized and converted into cellular components as the yeast cells grew, phytosterols concentration in SODD increased, and SODD was successfully concentrated. The optimal cultural medium of SODD was:SODD 40g/L, urea 1.0g/L, MgSO40.1g/L, K2HPO40.2g/L, KH2PO40.2g/L. The medium above was inoculated with Candida tropicalis1253, and the cultures were fermented at 30℃ in a shaker at 200r/min for96 h. When the fermentation was finished, consumption of fatty acids in SODD was 70.21%, and phytosterols concentration in SODD increased from 15.2% to 28.43%. Pretreatment of VODD by microbial fermentation was a technically feasible process and can replace pretreatment of methyl esterification of fatty acids in VODD.