荧光淬灭法测定三种蔬菜中铅(Ⅱ)含量

Fluorescence quenching method for the determination of plumbum (Ⅱ) in three kinds of vegetables

摘要: 酸性条件下,少量铅（Ⅱ）对氧氟沙星的荧光强度有猝灭作用,据此建立了一种测定铅（Ⅱ）含量的荧光光谱分析法。在优化的实验条件下,其荧光猝灭强度与铅（Ⅱ）浓度在3.0×10-7¹.0×10-5mol·L-1范围内呈良好的线性关系,其检出限为8.4×10-8mol·L-1,相对标准偏差为3.2%（c=1.0×10-6mol·L-1,n=11）。利用该法对三种蔬菜样品中铅（Ⅱ）含量的测定,回收率在94.46%¹01.5%之间。

Abstract: It was found that plumbum (Ⅱ) could quench the fluorescent intensity of ofloxacin in acid medium.Thus, a fluorescent spectral method for the determination of trace plumbum (II) was developed. Under the optimal experiment conditions, the fluorescent quenching intensity responded linearly with plumbum ( Ⅱ) concentration in the range of 3.0×10-7¹.0×10-5mol·L-1. And a detection limit of 8.4×10-8mol·L-1was estimated.The relative standard deviation (RSD) of this approach response to 1.0×10-6mol·L-1plumbum (Ⅱ) was 3.2% for11 successive measurements. This proposed method had been applied to detect the content of plumbum (Ⅱ) in three kinds of vegetable samples and the recovery was between 94.46% and 101.5%.