响应面法优化可口革囊星虫抗氧化多糖提取工艺研究

Optimization of extraction process by response surface methodology of anti-oxidant polysaccharides from Phasolosma esculenta

摘要: 本研究利用响应面法优化可口革囊星虫抗氧化多糖的提取工艺。在单因素实验的基础上,选取提取时间、提取温度、加酶量三个因素,根据Box-Benhnken中心组合实验设计原理,确定提取工艺的最佳条件。结果表明:可口革囊星虫多糖的最佳提取工艺条件为提取温度65℃,提取时间7h,加酶量10000U/g。在此条件下,星虫粗多糖的得率为0.879%±0.001%,DPPH·清除率为72.5%。

Abstract: Response surface methodology was employed to optimize conditions for the extraction of anti- oxidant polysaccharides from Phasolosma esculenta.Based on the single- factor tests, extraction time, extraction temperature and enzyme amount were selected out from extraction conditions for the three- level Box- Behnken experimental design.The results showed that optimal extraction process parameters were 65℃, 7h and 10000U /g.Under these conditions, the experimental yields of polysaccharides was 0.879% ± 0.001%, while DPPH·scavenging activity was 72.5%.