Abstract: Response surface methodology ( RSM) was used to optimize extraction of anthocyanin from Vitis vinifera suspension cultures.Based on single factor tests, acidic ethanol concentration ( containing 0.1% hydrochloric acid, V/V) , ultrasonic power, extraction time and extraction temperature were chosen as arguments and the content of anthocyanin as response value.The interaction of the respective variables and their influence on the content of anthocyanin and the optimum process were studied by using Box- Behnken central composite design and response surface analysis theory.The results showed that the optimum conditions were: liquid- solid ratio of 1∶9, acidic ethanol concentration 85%, ultrasonic power 300W, extraction time 38min and extraction temperature 42℃ for twice.Under these conditions, the content of anthocyanins was up to 3.96mg /L, which was consistent with the measured value ( 3.93mg /L) .The results indicated that ultrasound technology was suitable for the extraction of anthocyanins in Vitis Vinifera suspension cultures, and the statistical model fitting was with high reliability.This study has some practical significance for the research of the industrialized production of anthocyanins.