Abstract: Identification of wort turbid substances in Jiangsu Daner barley wort was performed.Results indicated that carbohydrates were the most abundant portion in wort turbid substances and the content of carbohydrates was82.57%, followed by proteins and polyphenols.Ion exchange chromatography was used to study the carbohydrates.It showed that the most abundant hydrolyzed monosaccharide was glucose, followed by galactose, arabinose and xylose.SDS- PAGE and MALDI- TOF/TOF were used to identify the protein components of wort turbid substances.Results revealed that the molecular weights of proteins were mainly 25 ~ 45ku and less than18.4ku and most of these proteins were derived from water-soluble proteins and ethanol-soluble proteins of malt.Mass spectrometry analysis showed that the maximum amount of haze protein was chain A of AMY2 /BASI complex, in addition to a small amount of protein Z4, γ3 hordein, caleosin 2 and BTI- CMe2.1.The results were instructive for the improvement of the quality of Jiangsu barley malt.