响应曲面法优化海红果水溶性多糖提取工艺及抗氧化活性的研究

贾琳斐; 郭姣; 李清宇; 范巧宁; 张伟刚; 彭晶; 段玉峰

doi:10.13386/j.issn1002-0306.2014.10.048

响应曲面法优化海红果水溶性多糖提取工艺及抗氧化活性的研究

Study on optimization of water-soluble polysaccharides extraction from Malus prunifolia (wild) Borkh using response surface methodology and its antioxidant activities in vitro

摘要

摘要: 采用响应曲面法优化海红果水溶性多糖（MPB）提取工艺的条件。在单因素实验基础上依据回归分析确定最佳工艺条件为:提取温度86℃,提取时间4h,料液比1∶28g/mL,MPB的实际提取率可达8.33%。用MPB对羟基自由基（·OH）、超氧阴离子自由基（O2-·）、DPPH自由基进行清除能力和还原力的测定,研究MPB的抗氧化能力。结果表明,MPB对·OH、O2-·、DPPH均有一定的清除能力,对DPPH的清除作用尤为明显,当质量浓度达到1mg/mL时,清除率达到87.12%,接近VC的94.47%,且MPB清除DPPH的IC50值为1.181mg/mL。

Abstract: Box-Behnken design was used to optimize the extraction conditions of water-soluble polysaccharides from Malus prunifolia (wild) Borkh. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The optimum extraction conditions were:optimized extraction time 4h, extraction temperature 86℃, and ratio of water to raw material 1∶28g/mL, the predicted yield of polysaccharides extracted was 8.33%. The antioxidant activity of the extraction was evaluated by scavenging hydroxyl radical (·OH) , superox ide an ion free radical (O2-·) , 1-diphenyl-2-picrylhydrazy (DPPH·) and reducing power. The in vitro antioxidant results showed that the inhibition effects of MPB on ·OH, O2-·, DPPH significant, MPB showed significant inhibitory effects on DPPH with IC50values of 1.181mg/mL, when the concentration of polysaccharides up to 1mg/mL, the scavenging capacity reached 87.12%, close to 94.47% by VC.