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中国精品科技期刊2020
王刚霞, 彭新媛, 吴忠红, 李维霞, 张正方, 吴斌. 核桃青皮提取物对几种采后病原真菌抑制作用的研究[J]. 华体会体育, 2014, (10): 142-145. DOI: 10.13386/j.issn1002-0306.2014.10.023
引用本文: 王刚霞, 彭新媛, 吴忠红, 李维霞, 张正方, 吴斌. 核桃青皮提取物对几种采后病原真菌抑制作用的研究[J]. 华体会体育, 2014, (10): 142-145. DOI: 10.13386/j.issn1002-0306.2014.10.023
WANG Gang-xia, PENG Xin-yuan, WU Zhong-hong, LI Wei-xia, ZHANG Zheng-fang, WU Bin. Inhibitory effect of extracts on several postharvest fungal diseases of walnut green husk[J]. Science and Technology of Food Industry, 2014, (10): 142-145. DOI: 10.13386/j.issn1002-0306.2014.10.023
Citation: WANG Gang-xia, PENG Xin-yuan, WU Zhong-hong, LI Wei-xia, ZHANG Zheng-fang, WU Bin. Inhibitory effect of extracts on several postharvest fungal diseases of walnut green husk[J]. Science and Technology of Food Industry, 2014, (10): 142-145. DOI: 10.13386/j.issn1002-0306.2014.10.023

核桃青皮提取物对几种采后病原真菌抑制作用的研究

Inhibitory effect of extracts on several postharvest fungal diseases of walnut green husk

  • 摘要: 采用生长速率法和孢子萌发法,研究了核桃青皮乙醇提取物对几种主要的采后致病真菌青霉菌(Penicillium citrinu)、匍枝根霉(Rhizopus stolonifer)、灰葡萄孢(Botryis cinerea)、镰刀菌(Fusarium oxysporum)和交链孢属(Alternaria)的抑制效应,确定其最低抑菌浓度和最低杀菌浓度。结果表明,核桃青皮提取物对供试菌种均有明显的抑制效果,提取物浓度为20mg/mL时,对5种供试菌种菌丝生长和孢子萌发的抑制率均达到88%以上。不同浓度提取物对相同病原菌的抑制效果差异显著(p<0.05),且随着浓度的增加抑制作用增强;提取物对5种孢子萌发的EC50值分别为:4.6384、10.2419、11.7834、8.3486和9.4516mg/mL,供试菌种菌丝的EC50值分别为:9.8142、11.6084、17.2737、10.1965和8.2892mg/mL。 

     

    Abstract: As this respect, the inhibitory effect of EEW for Penicillium citrinum, Rhizopus stolonifer, Botryis cinerea, Fusarium oxysporum, Alternaria were evaluated using mycelium growth rate and spore germination rate methods. The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were also assessed. The results showed that EEW could significantly inhibit the mycelium growth and spore germination of tested fungus. The antimicrobial activity of the extracts was shown by the samples (20mg/mL) , the mycelium growth and spore germination inhibition rate and the mycelium growth inhibition rate of four pathogens were all higher than 88%. Different concentrations had different activities to the same pathogenic fungus (p<0.05) . The EC50 values of EEW against the mycelium growth were 4.6384, 10.2419, 11.7834, 8.3486 and 9.4516mg/mL, spore germination of tested fungus were 9.8142, 11.6084, 17.2737, 10.1965 and 8.2892mg/mL, respectively.

     

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