Abstract: To study the extraction, purification and anti-inflammatory effect of total flavonoids from Acanthopananx Trifoliatus (L.) Merr. leaves, the orthogonal test was carried out to optimize the ultrasonic-assisted extraction method. 50 rats were divided into normal group, negative control group, positive control group, flavonoid lowdose group, flavonoid mid-dose group and flavonoid high-dose group. 7d after dosing by intra-peritoneal injection, carrageenan-induced hind paw edema in rats were conducted to investigate the anti-inflammatory effect. The content of total flavonoids in extraction with the optimized protocol was 37.01mg/g, and purity was up to 24.36% after purification. Total flavonoids at dose of 10, 20, 40mg/ (kg·d) could inhibite swelling induced by carrageenan. Results showed that total flavonoids from Acanthopanax trifoliatus (Linn.) Merr. leaves had anti-inflammatory effect, which further develop the medicinal value of Acanthopanax Trifoliatus (Linn.) Merr.