Abstract: The extraction process of tannin from Patrinia villosa Juss was studied through methods of organic solvent extraction and ultrasonic extraction. Extraction technology was optimized by single factor and orthogonal tests. Results showed that the ultrasonic extraction had higher extraction rate, and consumed less time. When the ultrasonic power was 80W, the ratio of material to solution was 1∶60 (w/v) and the extraction time was 60mins, the maximum extraction ratio was 4.684%.