单曲霉菌与混合曲霉菌发酵对纤溶酶活性的影响

Effect of single and mixed moulds fermentation methods on fibrinolytic activity

摘要: 以三株经过筛选诱变得到的纤溶酶高产突变株作为实验菌株,以大豆为原料,选取诱变后纤溶酶活性较大的黑曲霉3.4309进行单因素实验,以纤溶酶活性为指标,确定其产纤溶酶活性的最适发酵温度为30℃、接种量为10%、料水比0.6mL/g、装料量20g/250mL、pH5.5,发酵72h纤溶酶活性达到1.16TAME U/mL。在此条件下,运用混料设计确定最适的混菌接入量为黑曲霉3.4309添加4.3%、米曲霉3.800添加2.1%、米曲霉3.5232添加3.6%,72h发酵纤溶酶活性最大达到1.311TAME U/mL,比最优条件下单菌发酵纤溶酶活性高13%。

Abstract: Three screened and mutagenesised species of moulds was selected as trial strains, and were cultured in soybeans. Aspergillus niger 3.4309 was used to do single factor test for its higher fibrinolytic activity, using fibrinolytic activity as index, determine its optimum fermentation temperature was 30℃, inoculation amount was10%, the ratio of water was 0.6mL/g, loading capacity was 20g/250mL, the optimum pH was 5.5. Under these optimum conditons, the fibrinolytic activity could reach 1.16 TAME U/mL. Then used the mixture design method to determin the optimum mixed inoculation amount was:Aspergillus niger 3.4309 4.3% added, Aspergillus oryzae 3.800 2.1% added and Aspergillus oryzae 3.6% added. In this way, the fibrinolytic activity could reach1.311 TAME U/mL, 13% higher than fibrinolytic activity of single mould fermentation.