响应面优化酸热法从废弃黑曲霉提取壳聚糖工艺

Optimization acid-heating method for extracting chitosan from waste Aspergillus niger by using response surface methodology

摘要: 研究以酸热法破除黑曲霉细胞壁提取壳聚糖的工艺。在单因素实验基础上,运用Box-Behnken中心组合实验和响应面法考察了破壁温度、破壁时间和盐酸浓度3个因素对壳聚糖得率的影响,并优化了破壁工艺。结果表明,最佳破壁工艺为:破壁温度100℃,破壁时间1.6h,盐酸浓度2.1%,壳聚糖实际得率为10.87%,理论值11.06%,两者相对误差1.72%,说明响应面法优化酸热破除黑曲霉细胞壁工艺可行。

Abstract: This article discussed the practicability of acid-heating method which could break Aspergillus niger's cell walls in order to extract chitosan.Based on the single factor test analysis, the effects of three variables, which including cell wall breaking temperature, cell wall breaking time, and concentration of hydrochloric acid on the technology of breaking Aspergillus niger cell wall and the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis.The results indicated that cell wall breaking temperature of 100℃, cell wall breaking time of 1.6h, and concentration of hydrochloric acid of 2.1% were the optimum condition.The experimental values of chitosan yield were 10.87%, and the relative error with the theoretical value 11.06% was 1.72%. Therefore, the chitosan extraction from Aspergillus niger optimized by response surface methodology was very reliable.