豆豉中主要分离细菌对小鼠生化指标及肠道菌群的影响

董素琴; 武晓丽; 王报贵; 李胜杰; 王玉莲; 徐锋

doi:10.13386/j.issn1002-0306.2014.07.067

豆豉中主要分离细菌对小鼠生化指标及肠道菌群的影响

The effect of the main bacteria isolated from Douchi on biochemical indexes and the intestinal flora in mice

摘要

摘要: 采用豆豉样品进行细菌分离培养和细菌16S rDNA基因PCR扩增、测序分析,并将分离所得细菌进行小鼠灌胃实验。结果显示,豆豉中的主要分离细菌为枯草芽孢杆菌、解淀粉芽孢杆菌、类肠膜魏斯氏菌、乳酸片球菌、戊糖片球菌、发酵乳杆菌、表皮葡萄球菌。并且,各菌对小鼠丙氨酸转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、肌酐(CREA)、尿素氮(BUN)、尿酸(UA)、血糖(GLU)、甘油三酯(TG)、总胆固醇(TC)各项生化指标基本无影响。然而,其中解淀粉芽孢杆菌DC02能极显著性地降低小鼠血糖(GLU)水平,该菌有望成为治疗糖尿病的潜在菌株。活菌计数结果表明,除表皮葡萄菌以外,豆豉中分离所得其它细菌均能改善肠道菌群结构。可见,豆豉中分离所得主要细菌对正常机体安全、无致病性。

Abstract: Bacterial strains was isolated from Douchi and then 16S rDNA gene was amplifed. The bacterial strains was used for animal experiment in the method of intragastric administration after sequencing analysis. Results showed that the main bacteria in the Douchi was Bcillus subtilis, Bacillus amyloliquefaciens, Weissella paramesenteroides, Pdiococcus acidilactici, Pdiococcus pentosaceus, Lactobacillus fermentium and Staphylococcus epidermidis. Bacillus amyloliquefaciens could significantly decrease the level of blood glucose ( GLU) in mice after intragastric administration, and other bacteria had no effect on the level of alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , total bilirubin ( TBIL) , direct bilirubin ( DBIL) , creatinine ( CREA) , urea nitrogen ( BUN) , uric acid ( UA) , blood glucose ( GLU) , triglyceride ( TG) and total cholesterol ( TC) . The results of viable counting method revealed that bacteria strains isolated from Douchi could improve the structure of intestinal flora except Staphylococcus epidermidis.It follows that bacteria strains isolated from Douchi are safe for organism.