Abstract: The residue and disappearance law of melamine in blood, muscle and hepatopancreas organizations of Penaeus vannamei were studied at room temperature of 23 ~ 29℃ with containing different doses of melamine in feed food for 25d after domesticating 7d. The results showed that the melamine residues were existed in blood, muscle and hepatopancreas organizations, the highest residues ( 6.10mg /kg) of the melamine was in the hepatopancreas when the gavage dose of melamine was 800mg /kg. When the feed containing melamine was stopped, the melamine in blood, muscle and hepatopancreas were disappeared in 1, 4 and 6d.When the content of melamine reached 200 ~ 800mg /kg, the cyanuric acid was only detected in the hepatopancreas tissue, but not detected in the blood and muscle, the elimination time of cyanuric acid in hepatopancreas tissues was 3d. Therefore, the hepatopancreas can be used as target organ of the melamine residues in Penaeus vannamei.