Abstract: In order to find the strains which could reach high ethanol production as well as telerate inhibitors on the lignocellulose acid hydrolysates. Through sequentially increasing in the concentration of inhibitory compounds in five different acclimation media and acclimatizing 23 days, the yeast resistant to drug was obtained suitable to produce the ethanol from the lignocellulose acid hydrolyates.When the yeast resistant to drug and the parent strain grew in the same media which contained several inhibitory compounds 3.2g /L acetic acid, 0.8g /L furfural, 0.4g /L formic acid, the drug resistant yeast's maximal ethanol yield could reach 0.428g /g, up to 85.6% of theoretical ethanol yield.Compared with drug resistant yeast, the parent strain's maximal ethanol production yield only could reach 0.246g /g, up to 52.8% of theoretical ethanol yield. After 5 continuous passages, the average ability of producing ethanol was stable.Compared with parent strain, the yeast resistant to drug had good ability to ferment glucose and produce ethanol as well as tolerate inhibitors.