Abstract: 10 samples of Flos Lonicerae were tested by HPLC, which were from sichuan-chongqing. HPLC fingerprint of the methanol-dissolved elements provided a new way for scientific evaluation and effective quality control of Flos Lonicerae.The HPLC condition was that:Kromasil TM C 18 column, washing solution of methanol-1% phosphoric acid, 237nm wave length, 1.0mL /min flow rate, 30℃ column temperature. The result showed that:the common model of HPLC fingerprint was made and the similarity of Flos Lonicerae from different areas was compared, the various ingredients could be well separated from Sichuan-Chongqing Flos Lonicerae by this way, and the standard fingerprint was established with 11 characteristic fingerprint peaks. The HPLC fingerprint had a good similarity, reproducibility and characteristic.This study would be good for the standard planting and quality control of Flos Lonicerae in Sichuan-Chongqing region.