石莼多糖的单糖组成成分分析

冯学珍; 陈颖; 伍善广

doi:10.13386/j.issn1002-0306.2014.07.002

石莼多糖的单糖组成成分分析

Analysis of monosaccharide compositions in Ulva lactuca L polysaccharides

摘要

摘要: 目的:建立测定分析石莼多糖的单糖组成方法。方法:超声辅助提取石莼多糖,三氟乙酸（TFA）水解多糖,采用薄层色谱法（TLC）和1-苯基-3-甲基-5-吡唑酮（PMP）柱前衍生化高效液相色谱法（HPLC）测定单糖组成。结果:TLC法推断石莼多糖的单糖组成为甘露糖、核糖、鼠李糖、葡萄糖、半乳糖和木糖。PMP-HPLC法表明石莼多糖含有甘露糖、核糖、鼠李糖、葡萄糖、半乳糖和木糖,其摩尔比为:0.08∶0.08∶1.00∶0.97∶0.04∶0.68。结论:TLC法可以对多糖的单糖组成进行定性,但难以进行定量测定。PMP-HPLC法,操作简便,灵敏度高,分离效果好,可用于测定石莼多糖中单糖的组成定性、定量测定。

Abstract: Objective:To determine the monosaccharide compositions in polysaccharide from Ulva lactuca L polysaccharides.Methods:The polysaccharide was extracted by ultrasonic-assisted technology, and hydrolyzed with trifluoroacetic acid ( TFA) . The monosaccharide compositions were identified by TLC & PMP-HPLC. Results:The result of TLC analysis showed that the monosaccharide compositions were mannose, ribose, rhamnose, glucose, galactose and xylose.The result of HPLC analysis showed that the monosaccharide compositions were mannose, ribose, rhamnose, glucose, galactose and xylose and their molar ratio was about 0.08∶0.08∶1.00∶0.97∶0.04∶0.68. Conclusion:TLC method could be qualitative to monosaccharide composition of polysaccharides, but it was difficult to quantitatively measure.However PMP-HPLC was a simple method with high sensitivity, good resolution and integrity information.This method was simple, sensitive and accurate, and it could be used for determination of polysaccharide compositions in Ulva lactuca L, whether qualitative or quantitative.