毛霉高产中性蛋白酶发酵培养基的优化

刘莹莹; 刘颖; 张光; 孙冰玉; 王金凤; 石彦国

doi:10.13386/j.issn1002-0306.2014.06.032

毛霉高产中性蛋白酶发酵培养基的优化

Optimum fermentation medium of high-yielding neutral protease of mucor

摘要

摘要: 以雅致放射毛霉和五通桥毛霉为实验材料,采用单因素和正交优化实验对两种毛霉高产中性蛋白酶发酵培养基进行优化。结果表明:雅致放射毛霉产中性蛋白酶发酵培养基最佳成分为:蔗糖添加量为10%、黄豆粉添加量为2.5%、KH2PO4添加量为0.4%、CaCl2添加量为0.11%,此条件下中性蛋白酶酶活力达1310.56U/mL;五通桥毛霉产中性蛋白酶发酵培养基最佳成分为:蔗糖添加量为10%、黄豆粉添加量为3.0%、KH2PO4添加量为0.37%、CaCl2添加量为0.07%,此条件下中性蛋白酶酶活力达1306.38U/mL。两种毛霉酶活力提高的百分比分别为60.1%和46.7%,此研究成果为缩短腐乳后酵周期及直装腐乳发酵工艺的后续研究奠定了基础。

Abstract: Actinomucor elegans and Wutungkiao Mucor were used as experimental materials, through single factor and orthogonal experiments, the optimization of fermentation medium composition for high-yielding protease of two kinds of hair mould was researched. The results showed that the optimal fermentation medium composition for neutral protease of Actinomucor elegans was:sucrose content 10%, soybean flour 2.5%, KH2PO40.4%, CaCl20.11%, the neutral protease activity was 1310.56U/mL. The optimal fermentation medium composition for neutral protease of Wutungkiao Mucor was:sucrose content 10%, soybean flour 3.0%, KH2PO40.37%, CaCl20.07%, the last neutral protease activity was 1306.38U/mL. The improve percentage of neutral protease activities were 60.1% and 46.7% respectively. This research laid the foundation for follow-up study of shortenning the fermentation cycle of fermented bean curd and production process of direct-loading fermented bean curd.